Car (Carnosine) ELISA Kit – E-EL-0069

Car (Carnosine) ELISA Kit

The Car ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with Car. During the reaction, Car in the sample or standard competes with a fixed amount of Car on the solid phase supporterfor sites on the Biotinylated Detection Ab specific to Car. Excess conjugate and unbound sample or standard are washed from the plate, andAvidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Car in the samples is then determined by comparing the O.D. of the samples to the standard curve.<

Detected Target name: Car

Abbreviation : beta-alanyl-L-histidine

Uniprot ID :

Format : 96 rx

Reactivity : Universal (All Species expexted)

ELISA configuration : Competitive

Sensitivity : 8,438

Detection Range : 14.063-900 ng/mL

More information about this Car (Carnosine) ELISA Kit from Elabscience