Select Page

Calreticulin Antibody – MCA-6C6

  • Clonality : Mouse Monoclonal
  • Applications : WB | IF/ICC | IHC
  • Reactivity : Human | Rat | Mouse.

299.00$1,597.00$ CAD

Clear

Cashback Reward : Earn up to 159$!


Encor Mouse Monoclonal to calreticulin. 

Calreticulin was first identified as a calcium binding protein found in rabbit skeletal muscle. It appears to be a multifunctional protein found predominantly in endoplasmic reticulum (ER) and is ubiquitously expressed in a wide range of species (1). Gene knock out experiments show that the absence of the calreticulin gene is embryonically lethal (2). One of the major functions of calreticulin appears to be buffering ER Ca2+ levels in the ER which also affects intracellular calcium signalling (3). The N-terminal region of calreticulin has a high affinity calcium binding site, whereas the C-terminal region contains multiple low affinity calcium binding sites. Calreticulin also functions as an autophagy receptor and may also act as a molecular chaperone. Calreticulin, together with its membrane anchored homolog calnexin, assists proper folding of glycoproteins, prevent protein aggregation, and manage protein quality control (4). In addition to ER, calreticulin was found to localize to intracellular, cell surface, and extracellular compartments, from which calreticulin regulates a variety of important biological processes such as cell proliferation, cell adhesion, migration and cell anoikis. Calreticulin on the cell surface may mediate phagocytosis of apoptotic or dying tumor cells by triggering immune responses and allowing the immune system to remove tumor cells. This suggests that targeting cell surface calreticulin may be a novel anti-tumor therapy (5-7).
MCA-6C6 was raised against the synthetic peptide VESGSLEDDWDFLPPKKI corresponding to amino acids 191-208 of the human calreticulin protein. This peptide includes the LC3 interacting region or LIR motif of the molecule (8). This is the region which binds LC3 and other ATG8 family molecules, and this binding is important for autophagy. The HGNC name for this protein is CALR.


HGNC name(s) : CALR


Host : Mouse


Clonality : Monoclonal


ID : EnCor Biotechnology Calreticulin 6C6


Reactivity : Human | Rat | Mouse.


Isotype : IgG1


Conjugation : none


Immunogen : synthetic peptides VESGSLEDDWDFLPPKKI


Mass of detected protein : 48 kDa


Uniprot ID : P27797


KGNC name : CALR


RRID # : AB_2572240


Purification : Affinity purified at 1 mg/mL


Storage : Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.


Validated applications : WB | IF/ICC | IHC


Suggested Dilutions: 

WB: 1:1 000-5 000.
ICC/IF or IHC: 1:1 000-5 000.


Datasheet


Link to on EnCor’s website 


References :

1. Ostwald TJ, MacLennan DH. Isolation of a high affinity calcium-binding protein from sarcoplasmic reticulum. J Biol chem.10,249(3):974-9 (1974).

2. Mesaeli N, Nakamura K, Zvaritch E, Dickie P, Dziak E, Krause, K. H, Opas M, MacLennan DH, and Michalak M. Calreticulin is essential for cardiac development. J. Cell Biol. 144, 857–868 (1999).

3. Michalak M, Groenendyk J, Szabo E, Gold LI, and Opas M. Calreticulin, a multi-process calcium-buffering chaperone of the endoplasmic reticulum. Biochem. J. 417, 651–666 (2009).

4. Bedard K, Szabo E, Michalak M, and Opas M. Cellular functions of endoplasmic reticulum chaperones calreticulin, calnexin, and ERp57. Int. Rev. Cytol. 245, 91–121 (2005).

5. Obeid M, Tesniere A, Ghiringhelli F, Fimia G M, Apetoh L, Perfettini JL, Castedo M, Mignot G, Panaretakis T, Casares N, Metivier D, Larochette N, van Endert P, Ciccosanti F, Piacentini M, Zitvogel L, and Kroemer G. Calreticulin exposure dictates the immunogenicity of cancer cell death. Nat. Med. 13, 54–61 (2007).

6. Tesniere A, Apetoh L, Ghiringhelli F, Joza N, Panaretak T, Kepp O, Schlemmer F, Zitvogel L, and Kroemer G. Immunogenic cancer cell death: a key-lock paradigm. Curr.Opin. Immunol. 20, 504–511 (2008).

7. Gardai SJ, McPhillips KA, Frasch SC, Janssen WJ, Starefeldt A, Murphy-Ullrich JE, Bratton DL, Oldenborg PA, Michalak M, and Henson PM. Cell-surface calreticulin initiates clearance of viable or apoptotic cells through trans-activation of LRP on the phagocyte. Cell 123, 321–334 (2005).

8. Birgisdottir AB, Lamark T and Johansen T. The LIR motif – crucial for selective autophagy. J. Cell Sci. 126, 3237–3247 (2013).


Additional information

Format

50 ul, 100 ul, 500 ul

Supplier
Host
Clonality
Reactivity
Validated Applications
Conjugation
Isotype

Related Products

DNA Polymerase Comparison Chart

DNA Polymerase Comparison Chart

  Specifications and pricing of commercially available Taq DNA Polymerases and High-Fidelity DNA Polymerases in Canada.      

Detection Limit of High-Fidelity DNA Polymerases on gDNA

Detection Limit of High-Fidelity DNA Polymerases on gDNA

Published on Jan 30, 2017 by Simon Roy, Ph.D Detection Limit of High-Fidelity DNA Polymerases using Human gDNA for Template We previously determined the detection limit of different Taq DNA Polymerases using human genomic DNA as a template for PCR. For this instance,...

Product Reviews for Calreticulin Antibody – MCA-6C6

Reviews

There are no reviews yet.

Be the first to review “Calreticulin Antibody – MCA-6C6”

Your email address will not be published. Required fields are marked *

Order Today!

299.00$1,597.00$ CAD

Clear

Cashback Reward : Earn up to 159$!