c-Fos Antibody – RPCA-c-Fos-AP

Encor Rabbit polyclonal to c-Fos

c-Fos is a member of Fos family of transcription factors and is a cellular counterpart of the retroviral oncogene: v-Fos. Others member of Fos family is FosB, Fra-1 and Fra-2. Fos proteins associate with Jun family members, but also with other basic leucine-zipper (bZIP) proteins to create a variety of AP-1 (activator protein-1) complexes (1). Dimeric AP-1 complexes regulate major physiological processes such as cell proliferation, differentiation, neoplastic transformation, apoptosis, and response to stress.

c-Fos and c-Jun are the best-studied AP-1 components. They share a number of homologous domains, including adjacent basic and leucine zipper motifs, necessary for binding to DNA and dimerization, respectively. c-Fos and c-Jun-containing AP-1 dimers activate transcription by direct contacts with coactivators, such as the CBP (3), and constituents of the basal transcription machinery, such as the TATA-binding protein (4).

c-Fos is expressed constitutively in certain tissues. However, they are considered immediate-early genes because their expression is usually low but inducible rapidly and transiently in response to a wide array of stimuli including serum, growth factors, tumor promoters, cytokines, and UV radiation to allow cells to adapt to environmental changes. It plays an important role in many cellular functions and has been found to be overexpressed in a variety of cancers.

c-Fos is subjected to different modification: phosphorylation activates c-Fos, whereas sumoylation of c-Fos inhibits the AP-1 transcriptional activity (5,6). Several lines of data demonstrate expression of c-Fos by individual neurons can be used as a marker of cell activation, due to association of c-Fos expression with neurons fire action potentials (7,8,9,10). The HGNC name for this protein is FOS.

Antibody was raised in rabbit against full length recombinant protein expressed in and purified from E. coli. The antibody is affinity purified and diluted in PBS.

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HGNC name(s) : FOS

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Host : Rabbit

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Clonality : Polyclonal

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ID : EnCor Biotechnology c-Fos c-Fos-AP

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Reactivity : Human | Horse | Cow | Pig | Chicken | Rat | Mouse

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Isotype : IgG

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Conjugation : none

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Immunogen : Recombinant full length human

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Mass of detected protein : 50-65 kDa

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Uniprot ID : P01100

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KGNC name : FOS

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RRID # : AB_2572236

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Purification : Affinity purified at 1 mg/mL

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Storage : Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.

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Validated applications : WB | IF/ICC | IHC

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Suggested Dilutions:

WB: 1:1 000-1:2 000
ICC/IF or IHC: 1:5 000

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Datasheet

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Link to on EnCor’s website

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References :

1. Mildle-Langosch K. The Fos family of transcription factors and their role in tumourigenesis. European Journal of Cancer 41(16), 2449-2461 (2005).

2. Chiu R, Boyle WJ, Meek J, Smeal T, Hunter T, Karin M. The c-Fos protein interacts with c-Jun/AP-1 to stimulate transcription of AP-1 responsive genes. Cell 54 (4): 541–52. (1988)

3. Bannister AJ and Kouzarides T. CBP-induced stimulation of c-Fos activity is abrogated by ElA. The EMBO Journal 14 (19): 4758-4762 (1995).

4. Metz R, Bannister AJ, Sutherland JA, Hagemeier C, O’Rourke EC, Cook A, Bravo R, Kouzarides T. c-Fos-induced activation of a TATA-box-containing promoter involves direct contact with TATA-box-binding protein. Mol Cell Biol.14(9):6021-9 (1994).

5. Karin M. The regulation of AP-1 activity by mitogen activated protein kinases. J Biol Chem. 270(28):16483-6 (1995).

6. Bossis G, Malnou CE, Farras R, Andermarcher E, Hipskind R, Rodriguez M, Schmidt D, Muller S, Jariel-Encontre I, Piechaczyk M. Down-regulation of c-Fos/c-Jun AP-1 dimer activity by sumoylation. Mol Cell Biol.25(16):6964-79 (2005).

7. Day HE, Kryskow EM, Nyhuis TJ, Herlihy L, Campeau S. Conditioned Fear Inhibits c-fos mRNA Expression in the Central Extended Amygdala. Brain Res.1229: 137–46 (2008).

8. Hoffman G, Smith MS, Verbalis JG. c-Fos and related immediate early gene products as markers of activity in neuroendocrine systems. Fronties in Neuroendocrinology. 14(3):173-213 (1993 )

9. Van Elzakker M, Fevurly RD, Breindel T, Spencer RL. Environmental novelty is associated with a selective increase in Fos expression in the output elements of the hippocampal formation and the perirhinal cortex. Learn. Mem. 15 (12): 899–908 (2008).

10: Dragunow M, Faull R. The use of c-fos as a metabolic marker in neuronal pathway tracing. Journal of Neuroscience Methods 29 (3): 261–265 (1989).

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