BL21(DE3) pLysS Competent Cell – CD701

BL21(DE3) pLysS Chemically Competent Cell

BL21(DE3) pLysS Chemically Competent Cell is specifically designed for chemical transformation of DNA. It is resistant to chloramphenicol(Cam^R) and permits a transformation efficiency of over 10⁷ cfu/μg DNA (tested by pUC19 plasmid DNA). Control Plasmid I (Amp⁺) is used to detect cell expression function, the size of expressed protein is about 25 kDa.

Features of BL21(DE3) pLysS Competent Cells

• Transformation efficiency: >10⁷ cfu/μg (pUC19 DNA).
• Cam^R.
• Contains pLysS plasmid that expresses the T7 lysozyme gene to reduce the background of the target protein expression without disturbing IPTG induction.
• Suitable for non-toxic and toxic protein expression.
• Control plasmid I (Amp⁺) is used for detection of expression function of cell. The protein size is about 25 kDa.

Genotype

F-ompT hsdSB(rB-mB-) dcm(DE3) galλ pLysS(CamR)

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Product Manual and Datasheet

CD701 BL21 (DE3)pLysS Chemically Competent Cell