BL21 Chemically Competent Cell – CD901

BL21 Chemically Competent Cell

BL21 Chemically Competent Cell is specifically designed for chemical transformation of DNA. It is resistant to tetracycline (Tet^R) and their transformation efficiency is over 10⁷ cfu/μg DNA (tested by pUC19 plasmid DNA). Control Plasmid II (Amp⁺) is used to detect cell expression function. The size of expressed protein is about 26 kDa.

BL21 Chemically Competent Cell E. coli contains a chromosomal insertion of the gene encoding T7 RNA polymerase (T7RNAP) into the araB locus of the araBAD operon, placing regulation of T7 RNAP under the control of the arabinose-inducible araBAD promoter. Therefore, this strain is especially useful for the expression of toxic genes to other BL21 strains where basal expression of T7 RNAP is leaky. This BL21 strain does not contain the Ion protease and is deficient in the outer membrane protease OmpT. This genotype reduces degradation of heterologous proteins.

Features of BL21 cells

• Transformation efficiency: >10⁷ cfu/μg (pUC19 DNA).
• Tet^R.
• Tight expression control ideal for toxic protein expression.
• Control plasmid II (Amp⁺) is used for detection of expression function of cell. The protein size is about 26 kDa.

Genotype of BL21 cells

E. coli B F– dcm ompT hsdS(rB– mB–) gal araB::T7RNAP-tetA

Product Manual and Datasheet

CD901 BL21 Chemically Competent Cell