Select Page

Aurora A/B Kinase Antibody – MCA-5A12

  • Clonality : Mouse Monoclonal
  • Applications : WB | IF/ICC | IHC
  • Reactivity : Human | Horse | Cow | Pig | Chicken | Rat | Mouse

310.00$1,660.00$ CAD


Cashback Reward : Earn up to 166$!

Encor Mouse Monoclonal to Aurora A/B kinase. 

Aurora proteins are a family of serine/threonine protein kinases that play a key role in the regulation of cell division. The first Aurora kinase was discovered in Drosophila (1). Mutations of this kinase cause monopolar spindles surrounded by kinase, and the appearance of this was reminiscent of the Aurora borealis at the poles of the earth (1). Mammalian genomes encode 3 Aurora kinases named Aurora A, Aurora B, and Aurora C. All 3 contain a regulatory domain at the N terminus which is quite different between the molecules followed by a catalytic serine/threonine kinase domain which is almost identical between them.To download a sequence alignment of the 3 human Aurora proteins go here. As a consequence antibodies raised against one Aurora family member frequently cross-react with other family members. Since There is a short C-terminal peptide which is also variable between the three molecules (2). Aurora A is required for centrosome duplication, entry into mitosis, formation of bipolar spindlse and mitotic checkpoint (3). Aurora B is a chromosomal passenger protein and essential for chromosome condensation, kinetochore functions, spindle checkpoint activation and cytokinesis completion (4). Aurora C is heavily expressed in testis and is involved in spermatogenesis, but is also expressed in many cell lines and cancer cells and has been less well studied to date (5). Aurora A is first associated with centrosomes and then with spindle microtubules whereas Aurora B localizes to the spinal midzone and finally accumulates at the midbody. MCA-3H1 was raised against full length recombinant human Aurora A expressed in and purified from E. coli. The antibody was tested for binding to expressed human Aurora A, B and C and shown to react with both Aurora A and B, but not C (see Blot image). The HGNC names for Aurora A, B and C are AURKA, AURKB and AURKC. Since this antibody cross-reacted between Aurora A and B, we now have in progress monoclonal antibodies specific for only one Aurora gene product. The first of these is MCA-1A11, which is completely specific for Aurora A. MCA-3H1 is an excellent reagent for the visualization of mid bodies, centrosomes and spindles in dividing cells.

HGNC name(s) : AURKA

Host : Mouse

Clonality : Monoclonal

ID : EnCor Biotechnology Aurora A/B Kinase 5A12

Reactivity : Human | Horse | Cow | Pig | Chicken | Rat | Mouse

Isotype : IgG1

Conjugation : none

Immunogen : Recombinant full length human

Mass of detected protein : 46 kDa, 38 kDa

Uniprot ID : O14965, Q96GD4


RRID # : AB_2572231

Purification : Affinity purified at 1 mg/mL

Storage : Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.

Validated applications : WB | IF/ICC | IHC

Suggested Dilutions: 

WB: 1:1 000-1:2 000.
ICC/IF or IHC: 1:1 000-1:2 000.


Link to on EnCor’s website 

References :

1. Glover DM, Leibowitz MH, McLean DA, Parry H. Mutations in aurora prevent centrosome separation leading to the formation of monopolar spindles. Cell 81:95-105 (1995).

2. Hochegger H, Hegarat N, Pereira-Leal JB. Aurora at the pole and equator: overlapping functions of Aurora kinases in the mitotic spindle. Open Biol. Mar 20,3(3):120185 (2013).

3. Barr AR, Gergely F. Aurora-A: the maker and breaker of spindle poles. J Cell Sci.120:2987-96 (2007).

4. Andrew PD, Knatko E, Moore WJ, Swedlow JR. Mitotic mechanics: the auroras come into view. Curr Opin Cell Biol.15(6):672-83 (2003).

5. Tang CJ, Lin CY, Tang TK. Dynamic localization and functional implications of Aurora-C kinase during male mouse meiosis. Dev Biol. Dev Biol. 290(2):398-410 (2006).

Additional information


50 ul, 100 ul, 500 ul








Ask a question about Aurora A/B Kinase Antibody – MCA-5A12

Product Reviews for Aurora A/B Kinase Antibody – MCA-5A12


There are no reviews yet.

Be the first to review “Aurora A/B Kinase Antibody – MCA-5A12”

Your email address will not be published. Required fields are marked *

Related Products

More to Read

4th Anniversary Celebration

4th Anniversary Celebration

by Simon Roy | Sep 16th, 2019 | Latest News, Promotions | Promo code : civicbio4-50 1 per customer. Using this coupon will reduce Cashback Reward earning rate by 50%.  Until Nov, 30th, 2019 Double your Cashback Rewards with any product purchase! + Cashback x1.5...

PCR Purification Kit Comparison – DNA Clean Up

PCR Purification Kit Comparison – DNA Clean Up

Which is the Best Kit for DNA Clean Up? Our aim was to perform a PCR Purification Kit Comparison from different vendors. At Civic Bioscience, we distribute the Favorprep™ and EasyPure® product lines from FAVORGEN and TransBionovo respectively. First, the FavorPrep™...

The Truth about Antibodies used for Research

The Truth about Antibodies used for Research

The Problem with Antibodies used for Research Antibodies used for Research in academic, industrial and therapeutic fields are universal reagents in molecular biology assays. Without them, the entire field of medical research could collapse. However, there are many...

Detection Limit of Taq DNA Polymerase

Detection Limit of Taq DNA Polymerase

What's the Detection Limit of Taq ? How many DNA copies from gDNA? There are MANY Taq DNA Polymerases out there on the market. Some are driven by strong marketing and others by strong performance. Our aim was to verify the Detection Limit of Taq DNA Polymerases in PCR...

The 2019-2020 Price List is now available

A huge THANK YOU for your support! MERCI encore INFINIMENT de supporter notre jeune compagnie!   by Simon Roy | September 16th, 2019 | Company Posts, Latest NewsPrices for services vary case by case. 4rth anniversary promotions are coming soon! Let's CELEBRATE...