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Aurora A/B Kinase Antibody – MCA-5A12

  • Clonality : Mouse Monoclonal
  • Applications : WB | IF/ICC | IHC
  • Reactivity : Human | Horse | Cow | Pig | Chicken | Rat | Mouse

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Encor Mouse Monoclonal to Aurora A/B kinase. 

Aurora proteins are a family of serine/threonine protein kinases that play a key role in the regulation of cell division. The first Aurora kinase was discovered in Drosophila (1). Mutations of this kinase cause monopolar spindles surrounded by kinase, and the appearance of this was reminiscent of the Aurora borealis at the poles of the earth (1). Mammalian genomes encode 3 Aurora kinases named Aurora A, Aurora B, and Aurora C. All 3 contain a regulatory domain at the N terminus which is quite different between the molecules followed by a catalytic serine/threonine kinase domain which is almost identical between them.To download a sequence alignment of the 3 human Aurora proteins go here. As a consequence antibodies raised against one Aurora family member frequently cross-react with other family members. Since There is a short C-terminal peptide which is also variable between the three molecules (2). Aurora A is required for centrosome duplication, entry into mitosis, formation of bipolar spindlse and mitotic checkpoint (3). Aurora B is a chromosomal passenger protein and essential for chromosome condensation, kinetochore functions, spindle checkpoint activation and cytokinesis completion (4). Aurora C is heavily expressed in testis and is involved in spermatogenesis, but is also expressed in many cell lines and cancer cells and has been less well studied to date (5). Aurora A is first associated with centrosomes and then with spindle microtubules whereas Aurora B localizes to the spinal midzone and finally accumulates at the midbody. MCA-3H1 was raised against full length recombinant human Aurora A expressed in and purified from E. coli. The antibody was tested for binding to expressed human Aurora A, B and C and shown to react with both Aurora A and B, but not C (see Blot image). The HGNC names for Aurora A, B and C are AURKA, AURKB and AURKC. Since this antibody cross-reacted between Aurora A and B, we now have in progress monoclonal antibodies specific for only one Aurora gene product. The first of these is MCA-1A11, which is completely specific for Aurora A. MCA-3H1 is an excellent reagent for the visualization of mid bodies, centrosomes and spindles in dividing cells.


HGNC name(s) : AURKA


Host : Mouse


Clonality : Monoclonal


ID : EnCor Biotechnology Aurora A/B Kinase 5A12


Reactivity : Human | Horse | Cow | Pig | Chicken | Rat | Mouse


Isotype : IgG1


Conjugation : none


Immunogen : Recombinant full length human


Mass of detected protein : 46 kDa, 38 kDa


Uniprot ID : O14965, Q96GD4


KGNC name : AURKA


RRID # : AB_2572231


Purification : Affinity purified at 1 mg/mL


Storage : Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.


Validated applications : WB | IF/ICC | IHC


Suggested Dilutions: 

WB: 1:1 000-1:2 000.
ICC/IF or IHC: 1:1 000-1:2 000.


Datasheet


Link to on EnCor’s website 


References :

1. Glover DM, Leibowitz MH, McLean DA, Parry H. Mutations in aurora prevent centrosome separation leading to the formation of monopolar spindles. Cell 81:95-105 (1995).

2. Hochegger H, Hegarat N, Pereira-Leal JB. Aurora at the pole and equator: overlapping functions of Aurora kinases in the mitotic spindle. Open Biol. Mar 20,3(3):120185 (2013).

3. Barr AR, Gergely F. Aurora-A: the maker and breaker of spindle poles. J Cell Sci.120:2987-96 (2007).

4. Andrew PD, Knatko E, Moore WJ, Swedlow JR. Mitotic mechanics: the auroras come into view. Curr Opin Cell Biol.15(6):672-83 (2003).

5. Tang CJ, Lin CY, Tang TK. Dynamic localization and functional implications of Aurora-C kinase during male mouse meiosis. Dev Biol. Dev Biol. 290(2):398-410 (2006).


Additional information

Format

50 ul, 100 ul, 500 ul

Supplier

Host

Mouse

Clonality

Monoclonal

Conjugation

None

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