Select Page

Aldolase C: C terminal Antibody – MCA-1A1

  • Clonality : Mouse Monoclonal
  • Applications : WB | IF/ICC | IHC
  • Reactivity : Human | Horse | Cow | Pig | Chicken | Rat | Mouse

310.00$1,660.00$ CAD

Clear

Cashback Reward : Earn up to 166$!


Encor Mouse Monoclonal to Anti-Aldolase C: C-terminus specific. 

Aldolases are glycolytic enzymes that catalyze the reversible aldol cleavage of fructose 1,6-bisphosphate and fructose-1-phosphate to dihydroxyacetone phosphate and either glyceraldehyde 3-phosphate or glyceraldehyde, respectively. Three aldolase isozymes are found in mammals, specifically aldolases A, B, and C, each of which is encoded by a separate gene.
Aldolase A is generally considered to be a muscle enzyme. Northern analysis of cultured cells suggests that it is present in both neurons and glia (1). Aldolase B is considered to be a liver-specific enzyme and it is transcriptionally activated by signals from hormones and dietary factors (2). In the adult, aldolase C is the brain-specific isozyme, with low but detectable activity in fetal tissues (1, 3-6). Aldolase C shares 81% amino acid identity with aldolase A and 70% identity with aldolase B.

Earlier studies using isozyme-specific antibodies report its location in gray matter astrocytes and cells of the pia mater (5, 8). In situ hybridization of mouse central nervous system using isozyme-specific probes revealed that aldolase A and C are expressed in complementary cell types: aldolase A mRNA is found in neurons, aldolase C message is detected in astrocytes, some cells of the pia mater, and Purkinje cells (9). Aldolase C can in some situations be used as an astrocyte marker. However Purkinje cells of the cerebellum contain high levels of the enzyme, so the enzyme is not totally astrocyte specific.

MCA-1A1 was raised against C-terminal 23 amino acids of aldolase C protein, the sequence is KYEGSGEDGGAAAQSLYIANHAY. The HGNC name for this protein is ALDOC.


HGNC name(s) : ALDOC


Host : Mouse


Clonality : Monoclonal


ID : EnCor Biotechnology Aldolase C: C terminal 1A1


Reactivity : Human | Horse | Cow | Pig | Chicken | Rat | Mouse


Isotype : IgG1


Conjugation : none


Immunogen : C-terminal KYEGSGEDGGAAAQSLYIANHAY


Mass of detected protein : 40 kDa


Uniprot ID : P09972


KGNC name : ALDOC


RRID # : AB_2572224


Purification : Affinity purified at 1 mg/mL


Storage : Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.


Validated applications : WB | IF/ICC | IHC


Suggested Dilutions: 

WB: 1:1 000. IF/IHC: 1:500-1:1 000


Datasheet


Link to on EnCor’s website 


References :

1. Popovici T, Berwald-Netter Y, Vibert M, Kahn A, Skala H. Localization of aldolase C mRNA in brain cells. FEBS Lett. 268, 189-193 (1990).

2. Weber A, Marie J, Cottereau D, Simon M, Besmond C, Dreyfus J. & Kahn A. Dietary Control of Aldolase B and L-type Pyruvate Kinamse RNAs in Rat. J. Biol. Chem 259, 1798-1802 (1984).

3. Mukai T, Yatsuki H, Masuko S, Arai Y, Joh K & Hori K. The structure of the brain-specific rat aldolase C gene and its regional expression. Biochem. Biophys. Res. Commun. 174, 1035-1042 (1991).

4. Royds J, Ironside J, Warnaar S, Taylor C & Timperle W. Monoclonal antibody to aldolase C: a selective marker for Purkinje cells in the human cerebellum. Neuropathol. Appl. Neurobiol. 13, 11-21(1987).

5. Thompson R., Kynoch P. Willson V. Cellular localization of aldolase C subunits in human brain. Brain Res. 232, 489-493 (1982).

6. Schapira F, Reuber M, Hatzfeld A. Resurgence of two fetal-type of aldolases (A and C) in some fast-growing hepatomas. Biochem. Biophys. Res. Commun. 40, 321-327(1970).

7. Arai Y, Kajihara S, Masuda J, Ohishi S, Zen K, Ogata J. Mukai T. Position-independent, high-level, and correct regional expression of the rat aldolase C gene in the central nervous system of transgenic mice. Eur. J. Biochem. 221, 253-260 (1994).

8. Wachsmuth E, Thorner M. & Pfleiderer G. The cellular distribution of aldolase isozymes in rat kidney and brain determined in tissue sections by the immuno-histochemical method. Histochemistry, 45, 143-161 (1975).

9. Walther EU, Dichgans M, Maricich SM, Romito RR, Yang F, Dziennis S, Zackson S, Hawkes R, Herrup K. Genomic sequences of aldolase C (Zebrin II) direct lacZ expression exclusively in non-neuronal cells of transgenic mice. Proc Natl Acad Sci U S A. Mar 3,95(5):2615-20(1998).


Additional information

Format

50 ul, 100 ul, 500 ul

Supplier

Host

Mouse

Clonality

Monoclonal

Reactivity

Chicken, Cow, Horse, Human, Mouse, Pig, Rat

Validated Applications

WB, IHC, IF/ICC

Conjugation

None

Isotype

IgG1

Ask a question about Aldolase C: C terminal Antibody – MCA-1A1

Product Reviews for Aldolase C: C terminal Antibody – MCA-1A1

Reviews

There are no reviews yet.

Be the first to review “Aldolase C: C terminal Antibody – MCA-1A1”

Your email address will not be published. Required fields are marked *

Related Products

More to Read

4th Anniversary Celebration

4th Anniversary Celebration

by Simon Roy | Sep 16th, 2019 | Latest News, Promotions | Promo code : civicbio4-50 1 per customer. Using this coupon will reduce Cashback Reward earning rate by 50%.  Until Nov, 30th, 2019 Double your Cashback Rewards with any product purchase! + Cashback x1.5...

PCR Purification Kit Comparison – DNA Clean Up

PCR Purification Kit Comparison – DNA Clean Up

Which is the Best Kit for DNA Clean Up? Our aim was to perform a PCR Purification Kit Comparison from different vendors. At Civic Bioscience, we distribute the Favorprep™ and EasyPure® product lines from FAVORGEN and TransBionovo respectively. First, the FavorPrep™...

The Truth about Antibodies used for Research

The Truth about Antibodies used for Research

The Problem with Antibodies used for Research Antibodies used for Research in academic, industrial and therapeutic fields are universal reagents in molecular biology assays. Without them, the entire field of medical research could collapse. However, there are many...

Detection Limit of Taq DNA Polymerase

Detection Limit of Taq DNA Polymerase

What's the Detection Limit of Taq ? How many DNA copies from gDNA? There are MANY Taq DNA Polymerases out there on the market. Some are driven by strong marketing and others by strong performance. Our aim was to verify the Detection Limit of Taq DNA Polymerases in PCR...

The 2019-2020 Price List is now available

A huge THANK YOU for your support! MERCI encore INFINIMENT de supporter notre jeune compagnie!   by Simon Roy | September 16th, 2019 | Company Posts, Latest NewsPrices for services vary case by case. 4rth anniversary promotions are coming soon! Let's CELEBRATE...