8-OHdG (8-Hydroxydeoxyguanosine) ELISA Kit - E-EL-0028

8-OHdG (8-Hydroxydeoxyguanosine) ELISA Kit

The 8-OHdG ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with 8-OHdG. During the reaction, Universal 8-OHdG in the sample or standard competes with a fixed amount of 8-OHdG on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal 8-OHdG. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal 8-OHdG in the samples is then determined by comparing the O.D. of the samples to the standard curve.<

Detected Target name: 8-OHdG

Abbreviation : 8OHdG, 8-Oxo-DG, 7,8-Dihydro-8-Oxo-2′-Deoxyguanosine

Uniprot ID :

Format : 96 rx

Reactivity : Universal (All Species expexted)

ELISA configuration : Competitive

Sensitivity : 0,938

Detection Range : 1.563-100 ng/mL

More information about this 8-OHdG (8-Hydroxydeoxyguanosine) ELISA Kit from Elabscience