2′,3′-cyclic-nucleotide 3′-phosphodiesterase, CNP Antibody – Clone 1H10

Encor 2′,3′-cyclic-nucleotide 3′-phosphodiesterase, CNP Antibody – MCA-1H10

The 2′, 3′-Cyclic Nucleotide 3′-Phosphodiesterase (CNP), is an enzyme which catalyzes the hydrolysis of 2′, 3′-cyclic nucleotides to 2′-nucleotides, These cyclic nucleotides are structurally different from the better known and studied 3′-5′-cyclic nucleotides of which the best known example is cyclic AMP. The CNP enzyme hydrolyzes 2′,3′-cyclic nucleosidephosphates to produce nucleoside 2′-phosphates and water. CNP has two isoforms, CNPase 1 (46kDa) and CNPase 2 (48kDa), which are encoded separately by different promoters of the same gene (1). This enzyme is present in very high levels in brain and peripheral nerve, makes up 4% of total CNS myelin protein. It is found almost exclusively in oligodendrocytes and Schwann cells. It appears early in oligodendrocyte development, earlier than most other myelin proteins and continues to be expressed at high levels in these cells of adult animals (2). Antibody to CNP has been very useful as a marker for these particular cell types. CNP is thought to play a critical role in the events leading up to myelination, for the oligodendrocytes overexpressing CNP appear to mature earlier in development, resulting in earlier maximum gene expression for myelin basic proteins (3). It has been reported that CNP is also associated with microtubules in brain tissue and may promote microtubule assembly. CNP can link tubulin to cellular membranes, and may regulate cytoplasmic microtubule distribution (4). In various diseases, neurological mutants, and in experimental conditions in which myelin is reduced, CNP levels may also be severely reduced. Decreased brain levels of CNP have also been reported in Down syndrome and Alzheimer’s disease (5)., , This antibody was raised against full length recombinant human CNP expressed in and purified from E. coli. The HGNC name for this protein is CNP.

HGNC name(s) : CNP

Host : Mouse

Clonality : Monoclonal

Reactivity : Human | Rat | Mouse

Isotype : IgG1

Immunogen : Recombinant full length human

Mass of detected protein : 48 kDa

Uniprot ID : P09543

KGNC name : CNP

RRID # : AB_2572250

Purification : Affinity purified at 1 mg/mL

Storage : Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.

Validated applications : WB | IF/ICC | IHC

Suggested Dilutions:

WB: 1:5 000-20 000. IF/IHC: 1:1 000-5 000.

Datasheet

Link to 2′,3′-cyclic-nucleotide 3′-phosphodiesterase, CNP Antibody – MCA-1H10 on EnCor’s website

References :

1. Kretsinger RH & Nockolds CE. Carp Muscle Calcium-binding Protein: II. Structure determination and general description. J. Biol. Chem. 248:3313-3326 (1973).

2. Andressen C, Bliimcke I & Celio MR. Calcium-binding proteins: selective markers of nerve cells. Cell Tissue Res 271:181-208 (1993).

3. Schwaller B, Meyer M & Schiffmann S. ‘New’ functions for ‘old’ proteins: The role of the calcium binding proteins calbindin D-28k, calretinin and parvalbumin, in cerebellar physiology. Studies with knockout mice. The Cerebellum 1:241–258 (2002).