2′,3′-cyclic-nucleotide 3′-phosphodiesterase, CNP Antibody – CNP

Encor 2′,3′-cyclic-nucleotide 3′-phosphodiesterase, CNP Antibody – CPCA-CNP

The 2′, 3′-Cyclic Nucleotide 3′-Phosphodiesterase (CNP), is an enzyme which catalyzes the hydrolysis of 2′, 3′-cyclic nucleotides to 2′-nucleotides, These cyclic nucleotides are structurally different from the better known and studied 3′-5′-cyclic nucleotides of which the best known example is cyclic AMP. The CNP enzyme hydrolyzes 2′,3′-cyclic nucleosidephosphates to produce nucleoside 2′-phosphates and water. CNP has two isoforms, CNPase 1 (46kDa) and CNPase 2 (48kDa), which are encoded separately by different promoters of the same gene (1). This enzyme is present in very high levels in brain and peripheral nerve, makes up 4% of total CNS myelin protein. It is found almost exclusively in oligodendrocytes and Schwann cells. It appears early in oligodendrocyte development, earlier than most other myelin proteins and continues to be expressed at high levels in these cells of adult animals (2). Antibody to CNP has been very useful as a marker for these particular cell types. CNP is thought to play a critical role in the events leading up to myelination, for the oligodendrocytes overexpressing CNP appear to mature earlier in development, resulting in earlier maximum gene expression for myelin basic proteins (3). It has been reported that CNP is also associated with microtubules in brain tissue and may promote microtubule assembly. CNP can link tubulin to cellular membranes, and may regulate cytoplasmic microtubule distribution (4). In various diseases, neurological mutants, and in experimental conditions in which myelin is reduced, CNP levels may also be severely reduced. Decreased brain levels of CNP have also been reported in Down syndrome and Alzheimer’s disease (5)., , This antibody was raised against full length recombinant human CNP expressed in and purified from E. coli. The HGNC name for this protein is CNP.

HGNC name(s) : CNP

Host : Chicken

Clonality : Polyclonal

Reactivity : Human | Rat | Mouse

Isotype : IgY

Immunogen : Recombinant protein

Mass of detected protein : 48 kDa

Uniprot ID : P09543

KGNC name : CNP

RRID # : AB_2572249

Purification : Concentrated IgY preparation

Storage : Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.

Validated applications : WB | IF/ICC | IHC

Suggested Dilutions:

WB:1:5 000-10 000, IF/IHC: 1:5 000-10 000

Datasheet

Link to 2′,3′-cyclic-nucleotide 3′-phosphodiesterase, CNP Antibody – CPCA-CNP on EnCor’s website

References :

1. Janeway CA J, Travers P, Walport M et al. The complement system and innate immunity. Immunobiology: The Immune System in Health and Disease. 5th edition (2001).

2. Brade V, Hall RE, and Colten HR. Biosynthesis of pro-C3, a precursor of the third component of complement. J. Exp. Med. 146:759-765 (1977).

3. Xia Z, Stanhope KL, Digitale E, Simion OM, Chen L, Havel P, Cianflone K. Acylation-stimulating Protein (ASP)/Complement C3adesArg Deficiency Results in Increased Energy Expenditure in Mice* J Biol Chem. 279:4051-7 (2004) .

4. Maslowska M, Vu H, Phelis S, Sniderman AD, Rhode BM, Blank D, Cianflone K. “Plasma acylation stimulating protein, adipsin and lipids in non-obese and obese populations”. European Journal of Clinical Investigation 29:679–86.(1999)

5. Koistinen HA, Vidal H, Karonen SL, Dusserre E, Vallier P, Koivisto VA, Ebeling P. Plasma Acylation Stimulating Protein Concentration and Subcutaneous Adipose Tissue C3 mRNA Expression in Nondiabetic and Type 2 Diabetic Men”. Arteriosclerosis, Thrombosis, and Vascular Biology 21(6):1034–9 (2001).

6. Cianflone K, Zhang XJ, Genest J Jr, Sniderman A. Plasma acylation-stimulating protein in coronary artery disease. Arterioscler Thromb Vasc Biol.17:1239-44 (1997).

7. Wang Y, Hancock AM, Bradner J, Chung KA, Quinn JF, Peskind ER, Galasko D, Jankovic J, Zabetian CP, Kim HM, Leverenz JB, Montine TJ, Ginghina C, Edwards KL, Snapinn KW, Goldstein DS, Shi M, Zhang J. Complement 3 and Factor H in Human Cerebrospinal Fluid in Parkinson’s Disease, Alzheimer’s Disease, and Multiple-System Atrophy. The American Journal of Pathology, Vol. 178:1509-16 (2011)