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β-actin Monoclonal Antibody – E-AB-20092

  • Clonality : Mouse Monoclonal
  • Applications : WB
  • Reactivity : Human | Rat | Mouse | Monkey | Dog

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β-actin Monoclonal Antibody


Background information : Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins. 


Research area(s) : Epigenetics


Alternate names: ACTB, Actin, cytoplasmic 1, Beta-actin


Previous catalog number : ENM0133


Conjugation : none


Clonality : Mouse polyclonal


Clone number : 1B6


Isotype : IgG


Reactivity : Human | Rat | Mouse | Monkey | Dog


Validated applications : WB


Suggested dilutions : WB 1:500-1:2000


Immunogen : Synthetic Peptide


Swissprot ID : P60709


Gene ID / Accession : 60 /


Calculated MW : 42 kDa


Observed MW :


Purification method : Antigen affinity purification


Supplied in : PBS with 0.02% sodium azide, 50% glycerol pH7.4


Concentration : 1 mg/ml


Storage : Store at -20°C. Avoid freeze / thaw cycles.


Other informations :

Link to Elabscience product page


Manual & Datasheet


Additional information

Format

20 ul, 60 ul, 120 ul, 200 ul

Validated Applications

WB

Research Area(s)

Host

Mouse

Reactivity

Dog, Human, Monkey, Mouse, Rat

Product Name

?-actin Monoclonal Antibody

Supplier

Clonality

Monoclonal

Conjugation

None

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169.00$599.00$ CAD

Clear

Cashback Reward : Earn up to 59$!