β-actin Monoclonal Antibody – E-AB-20092
- Clonality : Mouse Monoclonal
- Applications : WB
- Reactivity : Human | Rat | Mouse | Monkey | Dog
169.00$ – 599.00$ CAD
β-actin Monoclonal Antibody
Background information : Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins.
Research area(s) : Epigenetics
Alternate names: ACTB, Actin, cytoplasmic 1, Beta-actin
Previous catalog number : ENM0133
Conjugation : none
Clonality : Mouse polyclonal
Clone number : 1B6
Isotype : IgG
Reactivity : Human | Rat | Mouse | Monkey | Dog
Validated applications : WB
Suggested dilutions : WB 1:500-1:2000
Immunogen : Synthetic Peptide
Swissprot ID : P60709
Gene ID / Accession : 60 /
Calculated MW : 42 kDa
Observed MW :
Purification method : Antigen affinity purification
Supplied in : PBS with 0.02% sodium azide, 50% glycerol pH7.4
Concentration : 1 mg/ml
Storage : Store at -20°C. Avoid freeze / thaw cycles.
Other informations :
Specifications and pricing of commercially available Taq DNA Polymerases and High-Fidelity DNA Polymerases in Canada.
Published on Jan 30, 2017 by Simon Roy, Ph.D Detection Limit of High-Fidelity DNA Polymerases using Human gDNA for Template We previously determined the detection limit of different Taq DNA Polymerases using human genomic DNA as a template for PCR. For this instance,...
Product Reviews for β-actin Monoclonal Antibody – E-AB-20092
169.00$ – 599.00$ CAD